ELISA antibody
  • • Bring microplate pouches to room temperature before opening.
  • • Level microwells evenly in microplate frame as the individual breakaway wells have very flexible plate frames leading to bowing off wells and yield poor washes.
  • • Place plates in dark immediately after addition of substrate solution, provided the substrate is sensitive to light.
  • • Grasp holder on grip marks when tapping to avoid strips slipping from holder.
  • • Rotate strips 180 and re-insert or use correct holder if strips do not fit in holder.
  • • Seal unused wells in purchase along with the desiccant.
  • • Date the pouches when first opened.
  • • Clean bottom surface of plates with wash buffer to remove fingerprints.
  • • Make sure microwells are at level during washing, reagent addition and plate/strip reading.
  • • Wipe the bottom the plate with a lint-free cloth/ towel before reading.
  • • Do not allow microwells to become dry once the assay has begun.

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