ELISA Tips-Temperature


  • Bring test reagents to room temperature (22-28 C) approximately 30 minutes prior to use.
  • Maintain proper incubation temperature:
    • Lower temperature can decrease OD values.
    • Higher temperatures can increase OD values.
    • Evaporation in wells can cause edging effect.
  • The optimal temperature for incubation is 22-28 C.
  • Check temperature against calibrated thermometer.
  • Strict adherence to time must be maintained.
  • Check calibration of timers.
  • Record time of incubation.
  • Read plate with specified time limits of adding stop solution.

 

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