ELISA Tips-Pipetting


  • Calibrate pipettes regularly according to manufacturer's instructions.
  • Avoid touching side wall of well with tips.
  • Avoid splashing of sample and reagents.
  • Avoid blowing out tip contents.
  • Use a new tip for each sample/control/reagent addition.
  • New tips should be used on the multichannel pipettes for each reagent to be added.
  • Reverse pipette when using the multichannel pipettes to add conjugate and substrate solution.
  • Forward pipette when using the multichannel pipettes to add stop solution.
  • Check pipette tips are long enough to provide air space between top of tip and pipette barrel.
  • Check pipette barrel for residual fluid of dried material, remove if present.
  • Ensure pipettes tips are fitted tightly.

 

Learn more: Washing tipsMicroplate tipsTemperature tipsIncubation tipsOther tips