Rabbit Monoclonal Antibody for ELISA

Advantages of Rabbit Monoclonal Antibody (RabMAb)

Monoclonal antibodies are traditionally produced by hybridoma - myeloma cells fused with the spleen cells, from a mouse which has acquired immunization of the specific antigen. It is cost-effective and easy to operate to use a mouse as a host to produce monoclonal antibodies. However, researchers have been confronted with a problem of mouse monoclonal antibodies - limitations. Firstly, many target proteins are highly conservative between mice and humans, and therefore, those proteins may be recognized as self-antigens by a mouse host, making the antigens less immunogenic than expected. Secondly, compared with rabbit monoclonal antibodies, mouse monoclonal antibodies have lower affinities, and thus have a weak attraction to bind to the target proteins. These problems can be avoided when using rabbit as a host animal. The availability of rabbit monoclonal antibodies is highly desirable for their high specificity and affinity, and greatly improved response to mouse antigens. Besides, rabbits have been known to produce many antibodies against those antigens that are not especially immunogenic in mice. Rabbit monoclonal antibodies also have other advantages, such as improved immune response to epitopes of low molecular weight proteins and more diverse epitope recognition, compared with mouse monoclonal antibodies. However, rabbit monoclonal antibodies production is much more difficult than that of mouse or rat monoclonal antibodies, due to lack of a stable fusion partner cell line for hybridoma development.